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Serum and plasma preparation

  Serum preparation  -Begin by collecting blood from animals with sterile syringes. - Place the blood in a clean tube and let it at room temperature for 15-30 minutes. - Place the tube in the centrifuge machine and spin it for 10-15 minutes at 3000 RPM to separate the serum from the blood cells. - After centrifuging the sample, the clot particles settle to the bottom and yellowish serum liquid comes on the surface.  - Extract the serum with a Pasteur pipette and preserve it for laboratory tests. plasma preparation When whole blood is collected in anticoagulant-treated tubes, plasma gets created. The blood does not clot in the plasma tube. Centrifugation is used to separate the cells. Using a Pasteur pipette, the supernatant, known as plasma, is carefully separated from the cell particle.
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Composition of blood- Flowchart

 

HOT TRENDING TOPIC “MURRHA” BECOMING POPULAR IN NEPAL:

        What does "Murrha" means? The unique breed known as the murrha was first discovered in the Indian state of Punjab. Murrha is world’s finest milk producing buffalo which is popular in dairy farming and also meat production. The average male and female body weights are 500–550 kg and 400–450 kg, respectively. First calving time is 42-43 month on average. It can yield 1550–2500 Ltr of milk each lactation. The fat proportion in milk is 7-8% and SNF (solid not fat) 10-12%. Why is murrha popular right now? A memorandum of agreement was agreed upon during Prime Minister Pushpa Kamal Dahal's recent visit to India to deliver the Murrah buffalo bulls to Nepal. A substantial increase in milk production will result from crossbreeding using artificial insemination on at least 100,000 buffaloes after the production of 150,000 doses of semen from 15 Murrah buffalo bulls in a year after three years, according to a statement released on Sunday by the Ministry of Agricul

Enterotoxemia: A common problems in Sheep and Goat

  ENTEROTOXEMIA ETIOLOGY • Caused by clostridium perfringens that produce toxin starting from A to F of which clostridium type A, B, C, D, E are important. INTRODUCTION It is an infectious disease of ruminant which is caused by clostridum perfringens gram positive bacteria which produce toxin. It`s  mainly effected maximum 2-10 month of animal.                                                   The bacteria of this disease are found in soil, feaces and intestine of ruminant. Excess milk feeding,grasses increase the number of bacteria which produce toxin that goes in blood and transfer to whole body of animal. TYPES OF CASUATIVE AGENTS 1.    Clostridium perfringens type-A: eexact role of type A in relation to disease in cattle,calves and lambs is not clear. It produce toxin.enterotoxaemic jaundice is seen in sheep and cattle.The affected animal show respiratory distress, bloody foam from mouth and nostrils. 2.                 Clostridium perfringens type-B: The organi

PHYSICS

 PHYSICS Chapter:- Radioactivity  Theory PDF file download here Topic included:- Definition and types of radioactivity, properties of alfa ray particles,  properties of beta rays particles,  properties of beta-gamma particles, Laws of radioactive disintegration, Decay equation, Half-life, Radiocarbon dating,  medical uses of radiation   Numerical PDF file download here Topic included:- Decay law of N, Relation between half-life and decay constant, Law of radioactivity. Chapter:- Photo-electricity PDF file   download   here Topic included:- Photo Electric effect, Quantum Theory of Radiation, Threshold energy or Work function (∅), Stopping Potential, Einstein’s Photo Electric Equation, Uses of the photoelectric effect, Bohr’s Theory of H- Atom, Numerical problems. Chapter:- Properties of Nucleus PDF file   download   here Topic included:- Nucleus, Types of Nucleus, Properties of Nucleus, Atomic mass unit (a.m.u) and its equivalent energy, Mass defect, Binding Energy, Binding Energy p

CHEMISTRY

DIPLOMA IN AGRICULTURE (FIRST YEAR)  1) CHEMISTRY= PDF DOWNLOAD Note:-          Topic included: Functional groups, Homologous series, IUPAC Nomencleature system, Alkanes,  2)CHEMISTRY=  PDF DOWNLOAD Note:-          Topic included: Ketones, Aldehydes, Ethers, Isomerism, Alkyl halides. 3) CHEMISTRY= PDF DOWNLOAD Note:-          Topic included: Organic Reaction, Bond fission, Inductive effect, Electrophiles and Nucleophiles, Resonance,